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qPCR Analysis Menu

Microdiagnostic analysis of key microbial species and enzymes provides insights into the progress of bioremediation strategies for site remediation. Search our qPCR targets below by contaminant, relevant remediation protocol and/or function. Then click the learn more button for further information on how each target can help with bioremediation planning, pilot optimisation and remediation monitoring of contaminated soil or water.

Number found:

41

Phenol monooxygenase

Target ID:

PMO

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Phenol

Contaminant degrading enzyme

PAH ring-hydroxylating dioxygenases (GP)

Target ID:

GP-PAH-RHD

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

PAHs (2-6 fused rings)

Contaminant degrading enzyme

Biphenyl dioxygenase gene

Target ID:

bhpA

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Polychorinated biphenyls (PCBs)

Contaminant degrading enzyme

Alkane monooxygenase A

Target ID:

almA

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Medium-long chain alkanes (C24-C38)

Contaminant degrading enzyme

Biphenyl dioxygenase (BphA)

Target ID:

bphA

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

BTEXN

Contaminant degrading enzyme

PAH ring-hydroxylating dioxygenases (GN)

Target ID:

GN-PAH-RHD

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

PAHs (2-6 fused rings)

Contaminant degrading enzyme

Toluene monooxygenase

Target ID:

TBM

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Benzene, Toluene

Contaminant degrading enzyme

Naphthalene dioxygenase

Target ID:

NDO

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Naphthalene

Contaminant degrading enzyme

Alkane monooxygenase B

Target ID:

alkB

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Short-medium chain alkanes (C6-C22)

Contaminant degrading enzyme

Toluene dioxygenase

Target ID:

TBD

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Benzene, Toluene, chlorobenzene

Contaminant degrading enzyme

Catechol 2,3-dioxygenase

Target ID:

C230

Process:

Aerobic hydrocarbon oxidation

Contaminant type:

Function of target:

BTEXNP

Contaminant degrading enzyme

Total Fungi

Target ID:

TotFung

Process:

All bioremediation processes

Contaminant type:

Function of target:

All biodegradable materials

Microbial abundance & health

Total Bacteria

Target ID:

TotBact

Process:

All bioremediation processes

Contaminant type:

Function of target:

All biodegradable materials

Microbial abundance & health

Total Archaea

Target ID:

TotArch

Process:

All bioremediation processes

Contaminant type:

Function of target:

All biodegradable materials

Microbial abundance & health

Benzylsuccinate synthase

Target ID:

BSS

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Toluene, Xylene

Contaminant degrading enzyme

Alkylsuccinate synthase

Target ID:

assA

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Medium-long chain alkanes (C24-C38)

Contaminant degrading enzyme

Sulphate-Reducing Bacteria

Target ID:

SRB

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Alkanes, alkenes, BTEXN and PAHs

Contaminant degrading species

Anaerobic benzene carboxylase

Target ID:

AbcAD

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Aromatics (Benzene, etc.)

Contaminant degrading enzyme

16S rRNA gene of Peptococcaceae

Target ID:

Pepto

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Benzene

Contaminant degrading enzyme

Ring-cleaving hydrolase

Target ID:

BamA

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Aromatics (Benzene, toluene etc.)

Contaminant degrading enzyme

Benzoyl-CoA reductase

Target ID:

BCR

Process:

Anaerobic hydrocarbon oxidation

Contaminant type:

Function of target:

Aromatics

Contaminant degrading enzyme

Nitrate reductase

Target ID:

napA

Process:

Denitrification

Contaminant type:

Function of target:

Nitrate

Contaminant degrading enzyme

Archaeal ammonia mono-oxygenase

Target ID:

AOA

Process:

Denitrification

Contaminant type:

Function of target:

Ammonia

Contaminant degrading enzyme

16S rRNA gene of Anammox

Target ID:

Anammox

Process:

Denitrification

Contaminant type:

Function of target:

Ammonia, Ammonium, Nitrite

Contaminant degrading species

Bacterial ammonia mono-oxygenase

Target ID:

AOB

Process:

Denitrification

Contaminant type:

Function of target:

Ammonia

Contaminant degrading enzyme

Methanogens

Target ID:

mcrA

Process:

Methanogenic degradation

Contaminant type:

Function of target:

Alkanes, alkenes, BTEXN and PAHs

Contaminant degrading species

Desulfitobacterium

Target ID:

DSB

Process:

Reductive dechlorination

Contaminant type:

Function of target:

PCE, TCE, 1,2-DCA

Contaminant degrading species

Dehalococoides

Target ID:

DHC

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Polychorinated biphenyls (PCBs)

Contaminant degrading species

Dehalogenimonas

Target ID:

DHG

Process:

Reductive dechlorination

Contaminant type:

Function of target:

PCE, TCE, 1,2-cisDCE, VC, 1,2-DCA, 1,2-DCP, and 1,1,2-TCA

Contaminant degrading species

Dehalobacter

Target ID:

DHB

Process:

Reductive dechlorination

Contaminant type:

Function of target:

PCE and TCE to cDCE. 1,1,1-TCA, 1,2-DCA, CF and DCM.

Contaminant degrading species

Sulphate-Reducing Bacteria

Target ID:

SRB

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Competes with ORB for hydrogen.

Degrading process competitors

Dehalococcoides

Target ID:

DHC

Process:

Reductive dechlorination

Contaminant type:

Function of target:

PCE, TCE, cDCE, tDCE & VC

Contaminant degrading species

Vinyl chloride reductase

Target ID:

bvcA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

DCE and VC to ethene

Contaminant degrading enzyme

Dehalogenimonas

Target ID:

DHG

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Polychorinated biphenyls (PCBs)

Contaminant degrading species

Chloroform reductase

Target ID:

tmrA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Chloroform (CF)

Contaminant degrading enzyme

Putative dechlorinating Chloroflexi

Target ID:

Chloroflexi

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Polychorinated biphenyls (PCBs)

Contaminant degrading species

Trichloroethene reductase

Target ID:

tceA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

TCE to VC

Contaminant degrading enzyme

Vinyl chloride reductase

Target ID:

vcrA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

VC to ethene

Contaminant degrading enzyme

1,2-dichloroethane reductive dehalogenase

Target ID:

dcaA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

1,2 Dichloroethane (DCA)

Contaminant degrading enzyme

Methanogens

Target ID:

mcrA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Competes with ORB for hydrogen.

Degrading process competitors

Reductive dehalogenase A

Target ID:

RdhA

Process:

Reductive dechlorination

Contaminant type:

Function of target:

Polychorinated biphenyls (PCBs)

Contaminant degrading enzyme

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