Archaeal ammonia mono-oxygenase

Item code:

Contaminant:

Remediation Protocol:

Target Type:

AOA

Ammonia

Denitrification

Contaminant degrading enzyme

qPCR Target Information

Ammonia monooxygenase (AMO) enzymes (particularly the amoA subunit) are among the most reliable molecular markers for identifying and quantifying ammonia-oxidising archaea (AOA) in environmental samples. Ammonia oxidation is the first and rate-limiting step in nitrification, where AOB convert ammonia (NH3) into nitrite (NO2-) via hydroxylamine. This process is catalysed by ammonia monooxygenase (AMO). Hence, AOB quantification is a robust indicator of nitrification potential rather than general microbial presence. Note, the same amoA subunit is targeted to quantify Bacterial ammonia monooxygenase (AOB).