qPCR Complete Pack List
Sulphate-Reducing Bacteria
SRB are widespread in the environment and play a key role in the anaerobic degradation of hydrocarbons, particularly in marine and petroleum-contaminated ecosystems. These bacteria utilise hydrocarbons as sources of carbon and energy, coupling their metabolism to the reduction of sulfate into sulfide.
16S rRNA gene of Anammox
Anammox bacteria (anaerobic ammonium-oxidizing bacteria) play a critical role in the global nitrogen cycle. These specialized microorganisms convert ammonium (NH4+) and nitrite (NO2-) directly into dinitrogen gas (N2) under anaerobic conditions, without generating nitrous oxide, a potent greenhouse gas. They are essential for maintaining nitrogen balance in oxygen-limited environments such as marine sediments, groundwater and wastewater systems. In engineered and natural systems, Anammox bacteria enable efficient nitrogen removal from wastewater and groundwater. Their activity can be enhanced through biostimulation with suitable electron donors (for example, emulsified vegetable oil), supporting sustainable, low-emission nitrogen treatment processes.
Methanogens
Methanogenic hydrocarbon biodegradation is an anaerobic microbial process in which methane is generated from the breakdown of hydrocarbons. This pathway plays an important role in the bioremediation of hydrocarbon-contaminated environments. The degradation can occur either through syntrophic interactions between hydrocarbon-degrading bacteria and methanogenic archaea or via direct methanogenesis by certain microbial communities.
Desulfitobacterium
Desulfitobacterium species are strictly anaerobic genus capable of reductive dehalogenation of a variety of chlorinated compounds including PCE, TCE, 1,2-DCA, chlorophenols and chlorobenzoate. Desulfitobacterium AusDCA is the key strain used in Micronovo's AusDCA high performance (low pH resistant) culture mix for complete dechlorination of DCA to ethene.
Dehalococoides
Dehalococoides (DHC) is an organohalide respiring species that are adept at dechlorinating PCBs under anaerobic conditions. Their reductive dehalogenase enzymes remove chlorine atoms from PCBs, making the PCBs less toxic. This process requires anaerobic conditions, hydrogen available for use as an electron donor and nutrients for optimal performance. This anaerobic process also makes the smaller/less chlorinated PCBs susceptible to aerobic oxidation by bacteria and fungi. Whilist initially developed for complete PCE and TCE dechlorination, the Dehalococcoides in AusPCE also dechlorinate PCBs.
Dehalogenimonas
Dehalogenimonas species are strictly anaerobic genus capable of reductive dehalogenation of a variety of chlorinated compounds including PCE, TCE, 1,2-cisDCE, VC, 1,2-DCA, 1,2-DCP, and 1,1,2-TCA.
Dehalobacter
Dehalobacter (DHB) is a strictly anaerobic genus which are capable of dechlorination of chlorinated ethenes (PCE and TCE to cDCE), chlorinated ethanes (1,1,1-TCA, 1,2-DCA to ethene) and chlorinated methanes (chloroform and dichloromethane) to acetate.
Dehalobacter UNSWDHB is the key strain used in Micronovo’s AusCF high performance organo-halide respiring bacteria culture mix for dechlorination of CF to DCM to harmless acetate.
Dehalococcoides
Dehalococcides (DHC) is a strictly anaerobic genus playing a key role in dehalogenation of chlorinated ethenes (PCE, TCE, cDCE, tDCE & VC). Dehalococcoides mccartyi are crucial to complete for the degradation of cDCE and VC to benign ethene. Dehalococcoides mccartyi are therefore key to the performance of Micronovo's AusPCE organo-halide respiring culture mix.
Dehalogenimonas
Dehalogenimonas species are strictly anaerobic and capable of reductive dehalogenation of a variety of chlorinated compounds including PCBs. They are positively correlated with PCB dechlorination and have been found in some studies to be codominanting over dehalococcoides.
Putative dechlorinating Chloroflexi
Chloroflexi, particularly specific genera within this phylum, are key organisms involved in the anaerobic dechlorination of polychlorinated biphenyls (PCBs) in contaminated sediments and soils. These bacteria use PCBs as electron acceptors, replacing chlorine atoms with hydrogen in a process known as reductive dechlorination. This transformation converts highly chlorinated, persistent PCB congeners into less chlorinated and typically less toxic forms, facilitating further degradation.
