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Bacterial ammonia mono-oxygenase
Ammonia monooxygenase (AMO) enzymes (particularly the amoA subunit) are among the most reliable molecular markers for identifying and quantifying ammonia-oxidising bacteria (AOB) in environmental samples. Ammonia oxidation is the first and rate-limiting step in nitrification, where AOB convert ammonia (NH3) into nitrite (NO2-) via hydroxylamine. This process is catalyzed by ammonia monooxygenase (AMO). Hence, AOB quantification is a robust indicator of nitrification potential rather than general microbial presence. Note, the same amoA subunit is targeted to quantify Archael ammonia monooxygenase (AOA).
Ammonia monooxygenase (AMO) enzymes (particularly the amoA subunit) are among the most reliable molecular markers for identifying and quantifying ammonia-oxidising bacteria (AOB) in environmental samples. Ammonia oxidation is the first and rate-limiting step in nitrification, where AOB convert ammonia (NH3) into nitrite (NO2-) via hydroxylamine. This process is catalyzed by ammonia monooxygenase (AMO). Hence, AOB quantification is a robust indicator of nitrification potential rather than general microbial presence. Note, the same amoA subunit is targeted to quantify Archael ammonia monooxygenase (AOA).